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non phosphorylated β catenin  (Cell Signaling Technology Inc)


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    Structured Review

    Cell Signaling Technology Inc non phosphorylated β catenin
    M-cadherin is required for the formation of adherens junctions and secondary myofibers during fetal development (A) Representative images and quantification of the area of the EDL muscle containing positive signal for pan-cadherin at E15 and E17.5 ( n = 3–4 WT mice and n = 3–4 CDH15 KO mice). (B) Representative images and quantification of the area of the EDL muscle containing positive signal for <t>non-phosphorylated</t> <t>β-catenin</t> at E15 and E17.5 ( n = 3–4 WT mice and n = 3–4 CDH15 KO mice). (C) Representative images and quantification of average myofiber size of the EDL muscle and the number of primary and secondary myofibers comprising the whole EDL muscle at E17.5 ( n = 4 WT mice and n = 4 CDH15 KO mice). Asterisks denote WT primary myofibers with multiple adjacent secondary myofibers or CDH15 KO primary myofibers with 0–1 adjacent secondary myofibers. Data are represented as mean ± SEM. ∗ p < 0.05; ∗∗∗ p < 0.001; ∗∗∗∗ p < 0.0001.
    Non Phosphorylated β Catenin, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 792 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 96 stars, based on 792 article reviews
    non phosphorylated β catenin - by Bioz Stars, 2026-10
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    Images

    1) Product Images from "M-cadherin regulates the formation of adherens junctions and secondary myofibers during fetal myogenesis to determine adult myofiber number and muscle mass"

    Article Title: M-cadherin regulates the formation of adherens junctions and secondary myofibers during fetal myogenesis to determine adult myofiber number and muscle mass

    Journal: Stem Cell Reports

    doi: 10.1016/j.stemcr.2026.102812

    M-cadherin is required for the formation of adherens junctions and secondary myofibers during fetal development (A) Representative images and quantification of the area of the EDL muscle containing positive signal for pan-cadherin at E15 and E17.5 ( n = 3–4 WT mice and n = 3–4 CDH15 KO mice). (B) Representative images and quantification of the area of the EDL muscle containing positive signal for non-phosphorylated β-catenin at E15 and E17.5 ( n = 3–4 WT mice and n = 3–4 CDH15 KO mice). (C) Representative images and quantification of average myofiber size of the EDL muscle and the number of primary and secondary myofibers comprising the whole EDL muscle at E17.5 ( n = 4 WT mice and n = 4 CDH15 KO mice). Asterisks denote WT primary myofibers with multiple adjacent secondary myofibers or CDH15 KO primary myofibers with 0–1 adjacent secondary myofibers. Data are represented as mean ± SEM. ∗ p < 0.05; ∗∗∗ p < 0.001; ∗∗∗∗ p < 0.0001.
    Figure Legend Snippet: M-cadherin is required for the formation of adherens junctions and secondary myofibers during fetal development (A) Representative images and quantification of the area of the EDL muscle containing positive signal for pan-cadherin at E15 and E17.5 ( n = 3–4 WT mice and n = 3–4 CDH15 KO mice). (B) Representative images and quantification of the area of the EDL muscle containing positive signal for non-phosphorylated β-catenin at E15 and E17.5 ( n = 3–4 WT mice and n = 3–4 CDH15 KO mice). (C) Representative images and quantification of average myofiber size of the EDL muscle and the number of primary and secondary myofibers comprising the whole EDL muscle at E17.5 ( n = 4 WT mice and n = 4 CDH15 KO mice). Asterisks denote WT primary myofibers with multiple adjacent secondary myofibers or CDH15 KO primary myofibers with 0–1 adjacent secondary myofibers. Data are represented as mean ± SEM. ∗ p < 0.05; ∗∗∗ p < 0.001; ∗∗∗∗ p < 0.0001.

    Techniques Used:

    Related Articles

    Immunocytochemistry:

    Article Title: Decoding the Molecular Mechanisms Underlying Electronic Cigarette Toxicity in Lung, Cancer and Stem Cells
    Article Snippet: .. Immunocytochemistry was performed using antibodies to EMT markers that included E- cadherin and vimentin (Millipore, Burlington, MA, USA), N- cadherin (R&D Systems, Minneapolis, MN, USA), metalloproteinase 9 (MMP9) and P120 (Abcam, Cambridge, MA, USA), and active (non-phosphorylated) β-catenin (Cell Signaling, Danvers, MA, USA). .. Also, an early endosome antigen 1 (EEA1) antibody (Cell Signaling, Danvers, MA, USA) was used for the Ecadherin co-localization study.

    Article Title: Epithelial-to-Mesenchymal Transition of A549 Lung Cancer Cells Exposed to Electronic Cigarettes
    Article Snippet: .. Immunocytochemistry was performed using antibodies to EMT markers that included E-cadherin and vimentin (Millipore, Burlington, MA, USA), N-cadherin (R&D Systems, Minneapolis, MN, USA), metalloproteinase 9 (MMP9) and P120 (Abcam, Cambridge, MA, USA), and active (non-phosphorylated) β-catenin (Cell Signaling, Danvers, MA, USA). .. Also, an early endosome antigen 1 (EEA1) antibody (Cell Signaling, Danvers, MA, USA) was used for the E-cadherin colocalization study.

    Article Title: Epithelial-to-Mesenchymal Transition of A549 Lung Cancer Cells Exposed to Electronic Cigarettes
    Article Snippet: .. Immunocytochemistry Immunocytochemistry was performed using antibodies to EMT markers that included E-cadherin and vimentin (Millipore, Burlington, MA, USA), N-cadherin (R&D Systems, Minneapolis, MN, USA), metalloproteinase 9 (MMP9) and P120 (Abcam, Cambridge, MA, USA), and active (non-phosphorylated) β-catenin (Cell Signaling, Danvers, MA, USA). .. Also, an early endosome antigen 1 (EEA1) antibody (Cell Signaling, Danvers, MA, USA) was used for the E-cadherin colocalization study.

    Western Blot:

    Article Title: Dishevelled-1 DIX and PDZ domain lysine residues regulate oncogenic Wnt signaling
    Article Snippet: .. Antibodies used for immunoblots are: DVL-1 (D3570; Sigma), HA (cst-3724; Cell Signaling), active/non-phosphorylated β-catenin (cst-8814; Cell Signaling), total β-catenin (sc-7963; Santa Cruz), DVL2 (sc-271319; Santa Cruz), DVL-3 (SAB4200007; Sigma), CK1Ɛ (sc-373912; Santa Cruz), Lamin A/C (cst-4777; Cell Signaling), β-tubulin (T7816; Sigma), and GAPDH (sc-47724; Santa Cruz), and β-actin (sc-47778; Santa Cruz). ..

    Immunofluorescence:

    Article Title: Diminution of STAT3 signaling inhibits vascular permeability and anaphylaxis
    Article Snippet: After washing with FACS buffer, cells were stained with phosphorylated STAT1-AF488 (Cat No. 612596; BD Biosciences) and phosphorylated STAT3-tyrosine705-AF647 (Cat No. 557815; BD Biosciences) for 30 minutes. .. Immunofluorescence Primary antibodies used were total β-catenin (Cat No. 2677; 1:100), active (non-phosphorylated) β-catenin (Cat No. 8814; 1:100), VE-Cadherin (Cat No.2500; 1:100) (Cell Signaling, Danvers, MA). ..

    Incubation:

    Article Title: Proliferation control of kidney interstitial cells
    Article Snippet: Cells were permeabilized with 0.5% Triton-X in PBS for 5 min, rinsed with PBS then incubated in Duolink PLA blocking solution (Sigma) for 1hr at 37°C. .. Cells were incubated in primary antibodies against Smad3 (Cell Signaling), Smad4 (Santa Cruz), TCF4 (Cell Signaling), active (non-phosphorylated) β-catenin (Cell Signaling), active (non-phosphorylated) β-catenin (Millipore) diluted 1:50 in Duolink Antibody Diluent at 4°C overnight then washed 2X 5 min in Duolink Wash Buffer A. ..



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    Image Search Results


    M-cadherin is required for the formation of adherens junctions and secondary myofibers during fetal development (A) Representative images and quantification of the area of the EDL muscle containing positive signal for pan-cadherin at E15 and E17.5 ( n = 3–4 WT mice and n = 3–4 CDH15 KO mice). (B) Representative images and quantification of the area of the EDL muscle containing positive signal for non-phosphorylated β-catenin at E15 and E17.5 ( n = 3–4 WT mice and n = 3–4 CDH15 KO mice). (C) Representative images and quantification of average myofiber size of the EDL muscle and the number of primary and secondary myofibers comprising the whole EDL muscle at E17.5 ( n = 4 WT mice and n = 4 CDH15 KO mice). Asterisks denote WT primary myofibers with multiple adjacent secondary myofibers or CDH15 KO primary myofibers with 0–1 adjacent secondary myofibers. Data are represented as mean ± SEM. ∗ p < 0.05; ∗∗∗ p < 0.001; ∗∗∗∗ p < 0.0001.

    Journal: Stem Cell Reports

    Article Title: M-cadherin regulates the formation of adherens junctions and secondary myofibers during fetal myogenesis to determine adult myofiber number and muscle mass

    doi: 10.1016/j.stemcr.2026.102812

    Figure Lengend Snippet: M-cadherin is required for the formation of adherens junctions and secondary myofibers during fetal development (A) Representative images and quantification of the area of the EDL muscle containing positive signal for pan-cadherin at E15 and E17.5 ( n = 3–4 WT mice and n = 3–4 CDH15 KO mice). (B) Representative images and quantification of the area of the EDL muscle containing positive signal for non-phosphorylated β-catenin at E15 and E17.5 ( n = 3–4 WT mice and n = 3–4 CDH15 KO mice). (C) Representative images and quantification of average myofiber size of the EDL muscle and the number of primary and secondary myofibers comprising the whole EDL muscle at E17.5 ( n = 4 WT mice and n = 4 CDH15 KO mice). Asterisks denote WT primary myofibers with multiple adjacent secondary myofibers or CDH15 KO primary myofibers with 0–1 adjacent secondary myofibers. Data are represented as mean ± SEM. ∗ p < 0.05; ∗∗∗ p < 0.001; ∗∗∗∗ p < 0.0001.

    Article Snippet: Primary antibodies for immunofluorescence staining were as follows: myosin heavy-chain isoforms (MHC type I, BA-D5; MHC type IIa, SC-71; MHC type IIx, 6H1; MHC type IIb, BF-F3; Developmental Studies Hybridoma Bank [DSHB], University of Iowa), laminin (L9393, Sigma or 1:500 ab11576, Abcam), dystrophin (ab15277, Abcam), myogenin (ab124800, Abcam), M-cadherin (611101, BD Biosciences), Pax7 (Pax7, DSHB), F-actin (1:500; a2228, Thermo Fisher Scientific), pan-cadherin (ab51034, Abcam), non-phosphorylated β-catenin (8814S, Cell Signaling Technology), and Ki67 (ab15580, Abcam).

    Techniques:

    G-1 promotes Wnt/Hedgehog-signaling in the human primary DPCs. (A) mRNA expression levels of Wnt signaling-related genes in human hair follicle dermal papilla cells (hDPCs) stimulated with G-1 with or without G-36 measured by qRT-PCR ( n = 6). Internal controls: RPLP0 expression. (B) Activation of the Wnt signaling-related protein β-Catenin in hDPCs stimulated with G-1, with or without G-36, measured using Western blotting ( n = 4). Internal controls: β-actin expression. (C) mRNA expression levels of Hedgehog signaling-related genes in hDPCs stimulated with G-1, with or without G-36, measured using qRT-PCR ( n = 6). Internal controls: RPLP0 expression. (D) Expression levels of Hedgehog signaling-related proteins in hDPCs stimulated with G-1, measured using Western blotting ( n = 8). Internal controls: β-actin expression. * p < 0.05, ** p < 0.01 (Tukey–Kramer's post hoc test). All data are presented as the means ± SE.

    Journal: Frontiers in Pharmacology

    Article Title: The GPR30 agonist G-1 promotes hair growth via Wnt/Hedgehog signaling in mice

    doi: 10.3389/fphar.2025.1570922

    Figure Lengend Snippet: G-1 promotes Wnt/Hedgehog-signaling in the human primary DPCs. (A) mRNA expression levels of Wnt signaling-related genes in human hair follicle dermal papilla cells (hDPCs) stimulated with G-1 with or without G-36 measured by qRT-PCR ( n = 6). Internal controls: RPLP0 expression. (B) Activation of the Wnt signaling-related protein β-Catenin in hDPCs stimulated with G-1, with or without G-36, measured using Western blotting ( n = 4). Internal controls: β-actin expression. (C) mRNA expression levels of Hedgehog signaling-related genes in hDPCs stimulated with G-1, with or without G-36, measured using qRT-PCR ( n = 6). Internal controls: RPLP0 expression. (D) Expression levels of Hedgehog signaling-related proteins in hDPCs stimulated with G-1, measured using Western blotting ( n = 8). Internal controls: β-actin expression. * p < 0.05, ** p < 0.01 (Tukey–Kramer's post hoc test). All data are presented as the means ± SE.

    Article Snippet: Western blotting was performed using primary antibodies specific for phosphorylated β-catenin (Ser33/37/Thr41) (1:1,000; Cell Signaling Technology, MA, United States), non-phosphorylated (active) β-catenin (1:1,000; Cell Signaling Technology), total β-catenin (1:1,000; Cell Signaling Technology), GLI1 (1:1,000; Cell Signaling Technology), and β-actin (1:1,000; Cell Signaling Technology).

    Techniques: Expressing, Quantitative RT-PCR, Activation Assay, Western Blot